Fig. 2. Isolated pancreatic islets and β-cells have an atypical ER leak that is independent of SERCA activity. (A,B) Percentage of ER Ca²⁺ leak, representing the maximal amount of Ca²⁺ released from the ER, under Ca²⁺-free conditions stimulated with the IP₃-generating agonists (A) carbachol (100 µM) in MIN-6 (red values) and INS-1 cells (green values) together with the SERCA-inhibitor BHQ (15 µM) or (B) histamine (100 µM) in HeLa (blue values) and EA.hy926 cells (black values) at the indicated time points. Bar charts represent the percentage of ER Ca²⁺ content after 20 min of incubation under Ca²⁺-free conditions. The corresponding 1 min value was set to 100% (n ≥ 6). *p<0.05 using one-way ANOVA.